Interaction

curated

Interaction Scheme

Molecule

Compound
Insuline human

c = 0.0 — 45.5 µM

Host

Scx6
sCx6

c = 50.0 µM

Cofactor

Dapi 2h
DAPI 2H+

c = 5.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.00⋅105 M-1
Kd =
logKa = 5.0
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -28.54 -6.82
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Associative Binding Assay
Technique: Fluorescence
𝛌ex = 370.0 nm
𝛌em = 455.0 nm
Detailed information about the solvation.
Solvent System Complex Mixture
Solvents water
Additives hydrochloric acid
pH 2.0
Please find here information about the dataset this interaction is part of.
Citation:

J. Mohanty, M. N. Shinde, N. Barooah, A. C. Bhasikuttan, SupraBank 2024, Inhibition and disintegration of insulin amyloid fibrils: a facile supramolecular strategy with p-sulfonatocalixarenes (dataset). https://doi.org/10.34804/supra.20210928181

Link: https://doi.org/10.34804/supra.20210928181
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

M. N. Shinde, N. Barooah, A. C. Bhasikuttan, J. Mohanty, Chem. Commun. 2016, 52, 2992–2995.

Link: https://doi.org/10.1039/c5cc10159j
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Insuline human (0.0002 M) and sCx6 (0 — 0.0004 M).