Interaction

curated

Interaction Scheme

Molecule

Artk%28me3%29qtarkstgy
ARTK(me3)QTARKSTGY

Host

Scx4
sCx4

Indicator

Lucigenin
Lucigenin

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 6.70⋅107 ± 4.40⋅106 M-1
Kd =
logKa = 7.83 ± 0.03
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -44.67 ± 0.16 -10.68 ± 0.04
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 369.0 nm
𝛌em = 485.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM phosphate pH-7.4
Solvents water 100.0 %
Additives Disodium phosph... 7.5 mM
Sodium dihydro... 2.5 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

M. Ali, K. D. Daze, D. E. Strongin, S. B. Rothbart, H. Rincon-Arano, H. F. Allen, J. Li, B. D. Strahl, F. Hof, T. G. Kutateladze, SupraBank 2024, Molecular Insights into Inhibition of the Methylated Histone-Plant Homeodomain Complexes by Calixarenes (dataset). https://doi.org/10.34804/supra.202109282

Link: https://doi.org/10.34804/supra.202109282
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

M. Ali, K. D. Daze, D. E. Strongin, S. B. Rothbart, H. Rincon-Arano, H. F. Allen, J. Li, B. D. Strahl, F. Hof, T. G. Kutateladze, Journal of Biological Chemistry 2015, 290, 22919–22930.

Link: https://doi.org/10.1074/jbc.M115.669333
Export: BibTex | RIS | EndNote

Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of ARTK(me3)QTARKSTGY (2.9850746268656716e-07 M) and sCx4 (0 — 5.970149253731343e-07 M).