Interaction

curated

Interaction Scheme

Molecule

Lucigenin
Lucigenin

c = 0.5 µM

Host

Scx4
sCx4

c = 0.0 — 16.87 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.26⋅107 ± 1.30⋅106 M-1
Kd =
logKa = 7.1 ± 0.04
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -40.53 ± 0.26 -9.69 ± 0.06
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 368.0 nm
𝛌em = 505.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM HEPES pH-7.4
Solvents water 100.0 %
Additives Hepes 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

D. Guo, J. Gao, Z. Zheng, W. Geng, H. Yu, Y. Wang, SupraBank 2024, Facile Fluorescence Monitoring of Gut Microbial Metabolite Trimethylamine N-oxide via Molecular Recognition of Guanidinium-Modified Calixarene (dataset). https://doi.org/10.34804/supra.20210928243

Link: https://doi.org/10.34804/supra.20210928243
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

H. Yu, W.-C. Geng, Z. Zheng, J. Gao, D.-S. Guo, Y. Wang, Theranostics 2019, 9, 4624–4632.

Link: https://doi.org/10.7150/thno.33459
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Lucigenin (1.5873015873015873e-06 M) and sCx4 (0 — 3.1746031746031746e-06 M).