Interaction Scheme

Molecule

Bc
Berberine chloride

c = 19.3 µM

Host

Scx8
sCx8

c = 0.0 — 315.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 3.09⋅105 ± 4.28⋅104 M-1
Kd =
logKa = 5.49 ± 0.06
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -31.34 ± 0.35 -7.49 ± 0.08
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 352.0 nm
𝛌em = 546.0 nm
Detailed information about the solvation.
Solvent System Buffer System phosphate pH-6.96
Solvents water
Additives Disodium hydrog... 3.7 mM
Potassium dihyd... 3.7 mM
Source of Concentration real
Total concentration 7.4 mM
pH 6.96
Please find here information about the dataset this interaction is part of.
Citation:

L. Biczók, M. Megyesi, SupraBank 2024, Considerable fluorescence enhancement upon supramolecular complex formation between berberine and p-sulfonated calixarenes (dataset). https://doi.org/10.34804/supra.2021092865

Link: https://doi.org/10.34804/supra.2021092865
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

M. Megyesi, L. Biczók, Chemical Physics Letters 2006, 424, 71–76.

Link: https://doi.org/10.1016/j.cplett.2006.04.016
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Berberine chloride (6.472491909385113e-05 M) and sCx8 (0 — 0.00012944983818770226 M).