Interaction Scheme

Molecule

Bc
Berberine chloride

c = 20.0 µM

Host

Beta cd
β-CD

c = 0.0 — 3000.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 87.0 ± M-1
Kd =
logKa = 1.94 ± 0.0
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -11.07 ± 0.0 -2.65 ± 0.0
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 348.0 nm
𝛌em = 530.0 nm
IboundIfree = 10.0
Detailed information about the solvation.
Solvent System Buffer System phosphate pH-7.2
Solvents water
Source of Concentration
pH 7.2
Please find here information about the dataset this interaction is part of.
Citation:

J. Yu, F. Wei, W. Gao, C. Zhao, SupraBank 2024, Thermodynamic study on the effects of β-cyclodextrin inclusion with berberine (dataset). https://doi.org/10.34804/supra.20210928305

Link: https://doi.org/10.34804/supra.20210928305
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

J.-S. Yu, F.-D. Wei, W. Gao, C.-C. Zhao, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2002, 58, 249–256.

Link: https://doi.org/10.1016/S1386-1425(01)00536-4
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Berberine chloride (0.22988505747126436 M) and β-CD (0 — 0.45977011494252873 M).