Interaction

uncurated

Interaction Scheme

Molecule

Mvcp
MVCP

c = 0.0 — 2.25 µM

Host

Cb8
CB8

c = 1.13 µM

Indicator

Bc
Berberine chloride

c = 122.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.68⋅1011 ± 2.00⋅1010 M-1
Kd =
logKa = 11.23 ± 0.05
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -64.07 ± 0.3 -15.31 ± 0.07
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 350.0 nm
𝛌em = 530.0 nm
Detailed information about the solvation.
Solvent System Buffer System 1X PBS pH-7.0
Solvents water
Additives sodium chloride 137.0 mM
Disodium hydrog... 10.0 mM
potassium chloride 2.7 mM
Potassium dihyd... 1.8 mM
Source of Concentration
Total concentration 151.5 mM
pH 7.0
Please find here information about the dataset this interaction is part of.
Citation:

L. Grimm, SupraBank 2024, Fluorescent Paracyclophanes: Unveiling Ultra-strong Binding with Cucurbit[8]uril in Aqueous Environments (dataset). https://doi.org/10.34804/supra.20240313527

Link: https://doi.org/10.34804/supra.20240313527
Export: BibTex | RIS | EndNote
Please find here information about the scholarly article describing the results derived from that data.
Citation:

Link: https://suprabank.org/datasets/527

Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of MVCP (1.1904761904761905e-10 M) and CB8 (0 — 2.380952380952381e-10 M).