Interaction

curated

Interaction Scheme

Molecule

Dapi 2h
DAPI 2H+

c = 5.0 µM

Host

Scx4
sCx4

c = 0.0 — 455.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2.80⋅104 M-1
Kd =
logKa = 4.45
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -25.38 -6.07
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 370.0 nm
𝛌em = 480.0 nm
Detailed information about the solvation.
Solvent System Single Solvent
Solvent water
pH 6.0
Please find here information about the dataset this interaction is part of.
Citation:

J. Mohanty, A. C. Bhasikuttan, M. N. Shinde, SupraBank 2025, Recognition-mediated contrasting fluorescence behaviour of 4′,6-diamidino-2-phenylindole (DAPI): probing the pKa of p-sulfonatocalix[4/6]arenes (dataset). https://doi.org/10.34804/supra.2021092816

Link: https://doi.org/10.34804/supra.2021092816
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

M. N. Shinde, A. C. Bhasikuttan, J. Mohanty, Supramolecular Chemistry 2016, 28, 517–525.

Link: https://doi.org/10.1080/10610278.2015.1126591
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of DAPI 2H+ (0.0007142857142857143 M) and sCx4 (0 — 0.0014285714285714286 M).