𝜈 | Molecule 1 : 1 Host | ||
Ka = | 5000.0 | ± 1000.0 | M-1 |
Kd = | |||
logKa = | |||
T | 25.0 °C | ||
Energy | kJ mol-1 | kcal mol-1 | |||
---|---|---|---|---|---|
ΔG | = | -21.11 | ± 0.5 | -5.05 | ± 0.12 |
Detection Method: | Direct | |||
Assay Type: | Associative Binding Assay | |||
Technique: | Fluorescence | |||
𝛌ex | = | 400.0 nm | ||
𝛌em | = | 450.0 nm |
Solvent System | Buffer System | 10 mM HEPES pH-7.0 |
Solvents | water | |
Additives | Hepes | 10.0 mM |
Source of Concentration | ||
Total concentration | 10.0 mM | |
pH | 7.0 |
Citation: |
W. M. Nau, F. Biedermann, A. Hennig, G. Ghale, SupraBank 2024, Fluorescent artificial receptor-based membrane assay (FARMA) for spatiotemporally resolved monitoring of biomembrane permeability (dataset). https://doi.org/10.34804/supra.20220628445 |
Link: | https://doi.org/10.34804/supra.20220628445 |
Export: | BibTex | RIS | EndNote |
Citation: |
F. Biedermann, G. Ghale, A. Hennig, W. M. Nau, Commun Biol 2020, 3, DOI 10.1038/s42003-020-1108-9. |
Link: | https://doi.org/10.1038/s42003-020-1108-9 |
Export: | BibTex | RIS | EndNote | |
The plot depicts the binding isotherm simulation of a 1:1 interaction of L-Phe (0.004 M) and CB8 (0 — 0.008 M).